A new qPCR method to detect Bonamia exitiosa in oysters from Foveaux Strait comprises a duplex qPCR assay for the co-amplification of the Bonamia target and the Ostrea chilensis β-actin gene, shortened lab procedures, and a block to allow effective co-amplification. This method has greater sensitivity and specificity than heart imprints (performance coefficient of 0.73 when compared to qPCR heart samples and 0.61 for gill samples), and is more cost-effective, but requires further development.
FAR 2015/39 New qPCR (quantitative polymerase chain reaction) and haematological sampling methods for monitoring Bonamia exitiosa infections and oyster (Ostrea chilensis) mortality in the Foveaux Strait fishery (OYU 5)
Report - Fisheries Assessment Report (FAR)
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